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1. 重庆医科大学附属第一医院皮肤性病科
2. 重庆医科大学附属第一医院皮肤性病科,重庆,400016
纸质出版日期:2007,
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[1]周汛,李桂明.110株念珠菌的菌种鉴定及药敏试验[J].重庆医科大学学报,2007(04):425-427.
ZHOU Xun, et al. Susceptibility testing and strain identification of Candida species[J]. Journal of Chongqing Medical University, 2007,(4):425-427.
目的:了解我院皮肤、粘膜念珠菌感染的菌种分布及其对两性霉素B、酮康唑、咪康唑、伊曲康唑、氟康唑、5-氟胞嘧啶及特比奈芬的体外敏感性。方法:收集临床110株念珠菌标本
采用科玛嘉显色培养基及API20C鉴定菌种
微量稀释念珠菌药敏试剂板检测其药物敏感性。结果:110株真菌中白色念珠菌94株
热带念珠菌6株
克柔念珠菌4株
光滑念珠菌4株
近平滑念珠菌2株。对酮康唑、咪康唑、伊曲康唑、氟康唑、5-氟胞嘧啶及特比奈芬的耐药率分别为0%、18.28%、1.81%、5.45%、3.63%及94.55%。结论:我院念珠菌感染仍以白念珠菌为主
对常用抗真菌药物呈不同程度的耐药
应建立真菌常规菌种鉴定及药敏试验
指导临床合理用药。
Objective:To identify the strain and to study the in-vitro susceptibilities of Candida species collected from clinical samples of skin and mucosa to Amphotericin B
Ketoconazole
Miconazole
Itraconazole
Fluconazole
5-Flucytosine and Terbinafin. Methods:110 clinical isolates of yeasts were identified by CHROMAGAR and API20C
meanwhile
the susceptibility to six antifungal drugs was examined by microdilution method using Sensititre Yeast One panels. Results:There were 94 C.albicans
6 C.tropicalis
4 C.krusei
4 C.glabrata
and 2 C.parapsilosis among 110 isolates of yeasts.There were 0.91%
1.81%
18.28%
1.81%
5.45%
3.63% and 94.55% of isolates displaying resistance to Amphotericin B
Ketoconazole
Miconazole
Itraconazole
Fluconazole
5-Flucytosine and Terbinafin
respectively.Conclusions:C.albicans is the main infection strain in our hospital
and candida species display different resistance to six antifungal drugs. It is necessary to test strain identification and susceptibility of candida species to guild the rational clinical drug use.
念珠菌菌种鉴定药敏试验
Candida speciesStrain identificationSusceptibility testing
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