脓毒症小鼠急性肾损害与TLR2受体基因表达的关系

朱方强; 江军; 黄宏

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脓毒症小鼠急性肾损害与TLR2受体基因表达的关系

更新时间：2024-01-17

- 脓毒症小鼠急性肾损害与TLR2受体基因表达的关系
- The relationship between gene expression of Toll-like receptor 2 and acute kidney lesion in mice with sepsis
- 重庆医科大学学报 2006年第5期页码：669-672
- 作者机构：
  
  1. 第三军医大学大坪医院泌尿外科
  2. 第三军医大学大坪医院泌尿外科,重庆,400042
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R459.7
- 纸质出版日期：2006，
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[1]朱方强,江军,黄宏.脓毒症小鼠急性肾损害与TLR2受体基因表达的关系[J].重庆医科大学学报,2006(05):669-672.

Zhu fangqiang, et al. The relationship between gene expression of Toll-like receptor 2 and acute kidney lesion in mice with sepsis[J]. Journal of Chongqing Medical University, 2006,(5):669-672.
[1]朱方强,江军,黄宏.脓毒症小鼠急性肾损害与TLR2受体基因表达的关系[J].重庆医科大学学报,2006(05):669-672. DOI：

Zhu fangqiang, et al. The relationship between gene expression of Toll-like receptor 2 and acute kidney lesion in mice with sepsis[J]. Journal of Chongqing Medical University, 2006,(5):669-672. DOI：

摘要

目的:探讨TLR2受体（Toll-like receptor 2）基因表达与严重感染所致急性肾损害的关系。方法:采用盲肠结扎穿孔术（cecal ligation puncture

CLP）复制小鼠脓毒症急性肾功能损害模型。将30只小鼠随机分为假手术组（对照组）和脓毒症组（实验组）

通过逆转录酶联反应（Reverse transcription and polymerase chain reaction

RT-PCR）检测肾组织TLR2mRNA表达

采用Western blot检测TLR2蛋白表达。采用ELISA法检测肾组织内肿瘤坏死因子α（TNFα）水平

同时测定血浆中肌酐（Cr）水平。结果:CLP术后8、12、24hTLR2mRNA表达均明显高于假手术组

TLR2蛋白表达与TLR2mRNA表达的变化规律相一致

均是12h达峰值

持续至伤后24h;肾组织TNFα水平和血清Cr水平于术后8h即显著升高达峰值

至24h

TNFα水平与假手术组无差异

而血清Cr仍维持高水平。并且TLR2 mRNA表达与肾组织TNFα和血清Cr水平呈显著正相关。结论:脓毒症时肾脏组织TLR2mRNA表达上调与急性肾损害密切相关。

Abstract

Objective:To observe the relationship of the expression of Toll-like 2 receptor(TLR2) with kidney injury in abdominal infection-induced sepsis.Methods:30 mice were randomly divided into 2 groups as follows: cecal ligation puncture group(CLP group

n=15) and sham operation group(control group

n =15).To adopt cecal ligation puncture(CLP) to establish the mice sepsis model

and the expression of TLR2 mRNA and TLR2 protein in kidney tissue were detected by reverse respectively.transcription and polymerase chain reaction(RT-PCR) and Western blo.The TNFαlevel in the kidney tissue and the creatinine(Cr)in the serum were also measured.Results:The expression of TLR2 mRNA in kidney tissues was markedly upregulated at 6h after CLP

peaked at 12h and kept in high level up to 24h

and the expression change of TLR2 protein was identical with the TLR2 mRNA expression.The expression of TLR2 mRNA was significantly correlated with TNFαand Cr levels.Conclusion:Severe infection could lead to marked up-regulation of TLR2mRNA and its protein expression in kidney tissue.There is a marked correlation between the changes of TLR2 expression and the actue kidney lesion.

关键词

脓毒症TLR2急性肾损害TNFα

Keywords

sepsisTLR2Acute kidney lesionTumor necrosis factorα(TNFα)

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