CD<sub>34</sub><sup>+</sup>细胞体外定向诱导分化的实验研究

姜蓉; 王建伟; 王亚平; 王莎莉

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CD₃₄⁺细胞体外定向诱导分化的实验研究

更新时间：2024-01-17

- CD₃₄⁺细胞体外定向诱导分化的实验研究
- Experimental study on induction of committed differentiation ofCD₃₄⁺ cells from human borrow cells in vitro
- 重庆医科大学学报 2006年第1期页码：38-41
- 作者机构：
  
  1. 重庆医科大学组织学与胚胎学教研室
  2. 重庆医科大学组织学与胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然科学基金（No.30472253）;重庆市科委重点攻关课题（渝科委计[2000]25）
- DOI：
  中图分类号： Q813
- 纸质出版日期：2006，
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[1]姜蓉,王建伟,王亚平,王莎莉.CD_(34)~+细胞体外定向诱导分化的实验研究[J].重庆医科大学学报,2006(01):38-41.

JIANG Rong, et al. Experimental study on induction of committed differentiation ofCD₃₄⁺ cells from human borrow cells in vitro[J]. Journal of Chongqing Medical University, 2006,(1):38-41.
[1]姜蓉,王建伟,王亚平,王莎莉.CD_(34)~+细胞体外定向诱导分化的实验研究[J].重庆医科大学学报,2006(01):38-41. DOI：

JIANG Rong, et al. Experimental study on induction of committed differentiation ofCD₃₄⁺ cells from human borrow cells in vitro[J]. Journal of Chongqing Medical University, 2006,(1):38-41. DOI：

摘要

目的:探讨造血生长因子不同组合方式对CD34+细胞体外定向诱导分化作用。方法:应用阴性分选策略

以Stem sepTM系统从正常人骨髓细胞中分离CD34+造血干/祖细胞（H SC/H PC）

在液体培养体系加入不同组合细胞因子对CD34+细胞进行诱导

检测细胞总数、CD71+细胞和CD15+细胞比例及粒单系祖细胞和红系祖细胞扩增数量。结果:液体培养体系中

以FL+Tpo+SCF+IL-3+Epo细胞因子组合对CD34+细胞向红系细胞的诱导分化能力最强

2w后CD71+细胞比例为61.20%±5.31%

BFU-E、CFU-E分别扩增27.12±3.95和30.65±40.26倍。以FL+Tpo+SCF+IL-3+GM-CSF+G-CSF细胞因子组合对CD34+细胞向粒系细胞的诱导分化能力最强

2w后CD15+细胞比例为56.18%±6.57%

CFU-GM扩增29.87±10.52倍。结论:合理组合生长因子

可定向诱导CD34+细胞生成大量血细胞

将有助于满足临床应用的需要。

Abstract

Objective: To investigate the role of recombinant human hematopoietic growth factors(HGFs) in committed differentiation of purified CD34+ cells derived from human borrow cells.Methods: A sterilized separation system for purification of CD34+ hematopoietic stem/progentior cells(HSC/HPC) was established by means of StemsepTM according to the strategy of negative selection on immunomagnetic separation.CD34+ cells were committedly differentiated with various combination of cytokines in liquid culture systems and the expanded cell number

CD33+ cell ratio

granucytopoietic progenitor cell and erythropoietic progenitor cell number were detected.Results: A combination of five HGFs including FL

Tpo

SCF

IL-3

and Epo was identified as the most potent combination inducing CD34+ cells to differentiate into erythropoietic cells.Two weeks later BFU-E and CFU-E were increased by 27.12±3.95 and 30.65±40.26 fold respectively;CD71+ cell rate was 61.20±5.31%;A combination of six HGFs including FL

Tpo

SCF

IL-3

GM-CSF and G-CSF was identified as the most potent combination inducing CD34+ cells to differentiate into granucytopoietic cells.CFU-GM was increased by 29.87±10.52 fold

and CD15+ cell rate was 56.18±6.57%.Conclusion: Appropriate HGFs combination may induce CD34+ cells to proliferate and differentiate into lots of blood cells.【

关键词

CD34+细胞诱导分化造血生长因子

Keywords

CD34+ CellsdifferentiationHematopoietic growth factor

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CD34+细胞体外定向诱导分化的实验研究

Experimental study on induction of committed differentiation ofCD34+ cells from human borrow cells in vitro

DOI：

摘要

Abstract

关键词

Keywords

references

CD₃₄⁺细胞体外定向诱导分化的实验研究

Experimental study on induction of committed differentiation ofCD₃₄⁺ cells from human borrow cells in vitro