Objective:To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods:Lysis buffer of different volume was taken to extract brain tissue proteins of rat

and different protein quantities(1mg

2mg

3mg) were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein

and patterns were analyzed.Results:With a molecular mass between 6.5~200ku and isoelectric points(pI) from 3~10

1mg proteins obtained 546 protein spots

2mg 780

and 3mg 805.Pattern of 2mg protein was the best.Conclusion:A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.