Objective:The purpose of this study is to determine if HIFU is feasible for the treatment of brain diseases by observing New Zealand Rabbits’ reaction

living status and variation in microscope after different dosages of ablation.Methods:By using the same total time of HIFU exposure

we took different divided time to sonicate localized brain tissues of 24 New Zealand Rabbits for several times

to observe the rabbits’ reaction and living status.All animals were sacrificed after 5 weeks.Then we applied TTC stain to observe the gross changes and HE stain to observe histological changes in microscope.Results:During the observation period

the rabbits’ living status was apparently getting worse when the single exposure was ≥ 20 second(s).The times of exposure were not correlated with living status.According to our observation

the rabbits could tolerate the dose of HIFU exposure <5W×20s

but histological and statistic study indicated that the best single exposure time was 5~10s.Conclusion:HIFU could ablate deep localized brain tissue with little effect on the superficial tissue.The toleration to HIFU exposure for brain tissue is correlated with single dose of sonication

but independent from times of exposure.