ATRA诱导HPV16型亚基因永生化人宫颈上皮细胞分化的实验研究

李祎铭; 赵涌; 林晓

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ATRA诱导HPV16型亚基因永生化人宫颈上皮细胞分化的实验研究

更新时间：2024-01-17

- ATRA诱导HPV16型亚基因永生化人宫颈上皮细胞分化的实验研究
- Experimental study of the differentiation of HPV16 subgenes-immortalized human endocervical cells induced by ATRA
- 重庆医科大学学报 2007年第1期页码：32-36
- 作者机构：
  
  1. 重庆医科大学基础医学院病理学教研室
  2. 重庆医科大学基础医学院病理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R737.33
- 纸质出版日期：2007，
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[1]李祎铭,赵涌,林晓.ATRA诱导HPV16型亚基因永生化人宫颈上皮细胞分化的实验研究[J].重庆医科大学学报,2007(01):32-36.

LI Yiming, et al. Experimental study of the differentiation of HPV16 subgenes-immortalized human endocervical cells induced by ATRA[J]. Journal of Chongqing Medical University, 2007,(1):32-36.
[1]李祎铭,赵涌,林晓.ATRA诱导HPV16型亚基因永生化人宫颈上皮细胞分化的实验研究[J].重庆医科大学学报,2007(01):32-36. DOI：

LI Yiming, et al. Experimental study of the differentiation of HPV16 subgenes-immortalized human endocervical cells induced by ATRA[J]. Journal of Chongqing Medical University, 2007,(1):32-36. DOI：

摘要

目的:研究ATRA对HPV16型亚基因（E6、E7）永生化人宫颈上皮细胞（H8细胞）的诱导分化作用及机制。方法:H8细胞经ATRA处理后

用MTT法测定ATRA的抗增殖效应

光镜、电镜观察细胞形态变化

流式细胞术（FCM）检测细胞周期

免疫组化SABC法检测增殖细胞核抗原Ki67表达水平的变化

PCR-ELISA法检测端粒酶活性变化

荧光免疫细胞化学检测HPV和E6蛋白表达变化。结果:ATRA使H8细胞增殖抑制;细胞形态朝良性方向分化;细胞堆积于G1期

S期细胞减少;细胞核内Ki67的表达水平下降;端粒酶活性下降。结论:ATRA对H8细胞有明显诱导分化作用

这种作用可能是通过抑制增殖

阻滞细胞周期

降低端粒酶活性来实现的。

Abstract

Objective:To study the differentiation-inducing effects of all-trans-retinoic acid (ATRA) on HPV16 subgenes-immortalized human endocervical cells (H8 cell) and the mechanism. Methods:After H8 cells were treated with ATRA

the aiti-proliferation of ATRA on them was tested by MTT assay

morphological changes observed by LM and TEM

cell cycle analyzed by FCM

expression of Ki67 tested by immunocytochemistry

the activity of telomerase tested by PCR-ELISA

and expressions of HPV and E6 tested by fluorescence immunocytochemistry. Results:ATRA could inhibit proliferation of H8 cells and induce their benign morphodifferentiation. According to FCM

H8 cells accumulated in G1 phase after being treated with ATRA

and expression of Ki67 and activity of telomerase reduced. Conclusion:ATRA could induce the differentiation of H8 cell line

which might be achieved by inhibiting proliferation

blocking cell cycle

and decreasing activity of telomerase.

关键词

维甲酸诱导分化宫颈HPV永生化

Keywords

Retinoic acidInduced-differentiationcervixHPVimmortalization

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