WANG Xin, et al. Determination of the content of ferulic acid in Xiaojin Wan soft capsule by HPLC[J]. Journal of Chongqing Medical University, 2007,(3):282-283.
WANG Xin, et al. Determination of the content of ferulic acid in Xiaojin Wan soft capsule by HPLC[J]. Journal of Chongqing Medical University, 2007,(3):282-283.DOI:
Objective:To establish a method for content determination of ferulic acid in Xiaojin Wan soft capsule by high performance liquid chromatography(HPLC).Methods:Analysis was carried out on Shimadzu LC-2010A HPLC.The separation was performed on DiamonsilTM C18 column(4.6×250mm
5μm)
with methanol:1% acetic acid(25:75) as mobile phase
the flow rate was 1.0ml/min
column temperature was 25℃ and the detection wavelength was 320nm.Results:A good linear relationship was found within the range of 3~18μg/ml
Regression equation was Y=100708.7X+24(r =0.9991)and the average recovery was 101.1%(RSD=1.86%
n=5).Conclusion:This method was highly sensitive
accurate and well represented
which could be used to determine the content of ferulic acid in Xiaojin Wan soft capsule.
关键词
小金丸软胶囊阿魏酸高效液相色谱法
Keywords
Xiaojin Wan soft capsuleFerulic acidhigh performance liquid chromatography(HPLC)