Objective:To investigate the effect and the mechanism of VEGF-C antisense oligodeoxynucleotide(ASODN) on the adhesion and invasion of human ovary cancer cell line SKOV3 in vitro.Methods:The ASODN sequence targeting the coding region of VEGF-C was synthesized and introduced into ovary cancer cells by liposomes.The expression of VEGF-C protein was detected by inmmnohistochemistry;the adhesion of SKOV3 cells to artifical basement membrane Matrigel was measured by MTT assay.The invasion was evaluated by transwell chambers attached with polycarbonate filters and reconstituted basement membrane(Matrigel).The expression of MMP-2 protein was measured by western blot.Result:In ASODN group

the adhesive rate and the invasive cell numbers were decreased markedly(P<0.01)compared with the control and sense oligodeoxynucleotide(SODN)group.The VEGF-C protein level was significantly decreased(P<0.01).The result of western blot showed a down-regulated MMP-2 expression(P<0.01).Conclusion:In vitro

the abilities of adhesion and invasion of ovary cancer cells can be suppressed by VEGF-C ASODN.Down-regulating the expressin of MMP-2 may be the mechanism.