CO<sub>2</sub>对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究

王华斌; 姚珍薇; 干晓琴; 李刚; 袁瑞; 乐爱文

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CO₂对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究

更新时间：2024-01-17

- CO₂对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究
- Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI
- 重庆医科大学学报 2007年第4期页码：384-386
- 作者机构：
  
  1. 重庆医科大学附属第一医院妇产科
  2. 重庆医科大学附属第一医院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R737.33
- 纸质出版日期：2007，
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[1]王华斌,姚珍薇,干晓琴,李刚,袁瑞,乐爱文.CO_2对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究[J].重庆医科大学学报,2007(04):384-386+391.

WANG Huabin, et al. Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI[J]. Journal of Chongqing Medical University, 2007,(4):384-386.
[1]王华斌,姚珍薇,干晓琴,李刚,袁瑞,乐爱文.CO_2对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究[J].重庆医科大学学报,2007(04):384-386+391. DOI：

WANG Huabin, et al. Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI[J]. Journal of Chongqing Medical University, 2007,(4):384-386. DOI：

摘要

目的:探讨腹腔镜二氧化碳（CO2）气腹对宫颈癌细胞体外生长的影响。方法:体外培养人宫颈癌细胞株Caski

以14mmHg气压的CO2处理2h

用四唑盐比色试验（MTT）、流式细胞术（FCM）和免疫细胞化学法检测Caski细胞外生长情况、细胞周期变化及周期相关蛋白CDK4和Cyclin D1的表达水平。结果:CO2处理后Caski细胞增殖加快

细胞周期中处于增殖期的细胞比例增高

周期相关蛋白CDK4和Cyclin D1的表达显著增加。结论:CO2可以促进Caski细胞的增殖。

Abstract

Objective:To study the effects of celioscope carbon dioxide pneumoperitoneum on the growth of cervical carcinoma cell line in vitro. Methods:Cervical carcinoma cells CASKI were cultured in vitro

and then treated with 14mmHg

100% carbon dioxide for 2h. The proliferation

cell cycle and expressions of CDK4 and Cyclin D1 were examined by MTT

FCM and immunocytochemistry respectively. Results:After the treatment of carbon dioxide

the proliferation of cervical carcinoma cells CASKI was significantly increased at the 3th day

the proportion of proliferative phase in the cell cycle was significantly increased

and the expressions of CDK4 and Cyclin D1 were also significantly increased. Conclusion:carbon dioxide may promote the proliferation of cervical carcinoma cell line.

关键词

二氧化碳宫颈癌细胞增殖

Keywords

Carbon dioxideCervical carcinoma cellproliferation

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CO2对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究

Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI

DOI：

摘要

Abstract

关键词

Keywords

references

CO₂对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究