盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响

章莉萍; 苏建华; 陈玉芳; 唐金荣; 肖杭; 包德诚

您当前的位置：

首页 >

文章列表页 >

盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响

更新时间：2024-01-17

- 盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响
- The effect of preconditioning with buflomedil hydrochloride on expression of Bcl-2 and Bax on neuron cells of DRG after the sciatic nerve lesion model in rats
- 重庆医科大学学报 2007年第4期页码：359-361
- 作者机构：
  
  1. 金坛市人民医院儿科
  2. 金坛市人民医院神经内科
  3. 常州市第四人民医院病理科
  4. 南京医科大学附属第一医院神经内科
  5. 南京医科大学应用神经毒理研究所
- 作者简介：
- 基金信息：
  
  江苏省常州市卫生局资助项目（编号:2004182）。
- DOI：
  中图分类号： R965
- 纸质出版日期：2007，
扫描看全文
[1]章莉萍,苏建华,陈玉芳,唐金荣,肖杭,包德诚.盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响[J].重庆医科大学学报,2007(04):359-361.

ZHANG Liping, et al. The effect of preconditioning with buflomedil hydrochloride on expression of Bcl-2 and Bax on neuron cells of DRG after the sciatic nerve lesion model in rats[J]. Journal of Chongqing Medical University, 2007,(4):359-361.
[1]章莉萍,苏建华,陈玉芳,唐金荣,肖杭,包德诚.盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响[J].重庆医科大学学报,2007(04):359-361. DOI：

ZHANG Liping, et al. The effect of preconditioning with buflomedil hydrochloride on expression of Bcl-2 and Bax on neuron cells of DRG after the sciatic nerve lesion model in rats[J]. Journal of Chongqing Medical University, 2007,(4):359-361. DOI：

摘要

目的:探讨盐酸丁咯地尔对周围神经损害后神经元保护机制。方法:采用SD大鼠

随机分为正常组、模型组、预处理组（盐酸丁咯地尔组）

预处理14天后制作坐骨神经损害动物模型

分别在模型制备后6h、12h、24h、48h、72h、96h、7天各组提取L1～5背根神经节作免疫组化计数Bcl-2、Bax阳性细胞表达数

并加以组间比较。结果:（1）预处理组于模型制备后12hBcl-2即有明显表达

24～48h达高峰

持续7天时与模型组比较仍有显著性差异（P<0.05）;（2）预处理组Bax蛋白表达与同期模型组均有不同程度降低

7天时表达到最低值

与模型组比较有显著性差异（P<0.05）。结论:盐酸丁咯地尔的神经元保护作用与其上调bcl-2蛋白、抑制Bax蛋白的表达有关。

Abstract

Objective:To explore protective mechanism of buflomedil Hydrochloride on the neuron of damaged Sciatic nerve in rats. Methods:SD rats were divided into normal

model

buflomedil hydrochloride group in random. Preconditioned for 14 days

the sciatic nerve lesion models were performed

the Bcl-2 and Bax positive cells were counted by immmunohistochemistry at the end of 6h

12h

24h

48h

72h

96h

7days respectively. Results:(1) At the end of 12h after the model perfomed

The expressions of Bcl-2 in buflomedil hydrochloride group increased obvionsly than the normal and model groups

peaked at 24~48h

and at the end of 7days

the expressions of Bcl-2 in buflomedil hydrochloride group increased obviously compared with model group.(2)The expressions of Bax in buflomedil hydrochloride groug were lower than the normal and model groups in the same periods

and that was the lowest at the end of 7 days

It was different significantly than that of model group(P<0.05). Conclusion:Buflomedil hydrochloride might upregulated the expressions of Bcl-2 and downregulated the expressions of Bax in neuron damaged sciatic nerve

which might be contributed to their protective effects.

关键词

盐酸丁咯地尔预处理神经元Bcl-2Bax大鼠

Keywords

Buflomedil HydrochloridepreconditioningneuronBcl-2Baxrats

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

survivin在脑胶质瘤中的表达及其与bcl-2、bax相关性的研究

五溴联苯醚影响乳腺癌MCF-7细胞增殖和凋亡的机制探讨

皮质发育障碍模型鼠海马区神经元凋亡机制的研究

细胞色素C对大鼠睡眠呼吸暂停综合征模型的作用

CCK-8通过上调Bcl-2/Bax的比率和下调Caspase-3的表达降低谷氨酸诱导的皮层神经元凋亡