Objective:To study the effect of varicocele(VC)on Expression of hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in germ cells of adolescent rats

and investigate the mechanism of the infertility resulting from varicocele.Methods:The varicocele model was created by partial ligation of the left renal vein in adolescent male Wistar rats

a total of 50 rats were randomly divided into four groups

namely varicocele group

sham operation group(SOG)

At 30 days and 60 days were completed

left orchiectomy was performed the expression of HIF-1α and VEGF were detected by immunohistochemical method.Results:The expression of HIF-1α and VEGF in left testes of varicocele group is significantly higher than of sham operation group.With the duration of varicocele mode1

HIF-1α and VEGF expression in experiment group was decreased

but was higher compared with that in control group.Conclusion:Experimental varicocele could change Expression of hypoxia-inducible factor-1α(HIF-1α)and VEGF in germ cells of adolescent rats.