Objective:To investigate the cultivation and boilogical char-acter of corneal epithelial cells(ECs) and oral ECs planted on amniot-ic membrane (AM) as a substrate. Methods:Rabbit limbus ECs were planted on denuded AM and intact AM respectively. Oral Ecs were p-lanted on denuded AM.The cultivated epithelial sheet was examined by HE staining pathological section and electronmicroscopy(EM)

also immunohistochemically labeled with AE5 monoconal antibody to identify the expression of cytokeratin 3(CK3). Results:Rabbit corneal ECsgrew slowly and were difficult to stick and confluence on intact AM

while both rabbit corneal and oral ECs were easy to grow and proli-ferate on denuded AM wheather using tissue pieces or single cells.T-he resulted tissure was composed of the basement membrane of AM and multiple layers of ECs showing the presence of CK3. Conclusions:The stratification of corneal and oral epithelium layers attaching to the basement membrane of AM are similar to engineering tissue mak-ing a fundament to ocular surface reconstruction.