Objective:To investigate the protective effect of ginkgetin from oxidative stress on immature testis induced by cyclophosphamide(CP) metabolite acrolein(ACR) in vitro.Methods:The sertoli cells were isolated from 7-days old SD rats’ testes

culture and passage to F2 generations.Experiment groups were divided into: 1)Ginkgetin incubated for 12 hours and then treat with ACR 2) Ginkgetin and ACR add to cells at the same time 3) Ginkgetin and ACR were incubated for 12 hours

then add to cells.Control group include: 1) Vitamin E were incubated cells for 12 hours

then add ACR respectively 2) Added ACR only as the negative control group 3) Not add any factors as the positive control group.3 h later the viability of sertoli cell were determined by MTT.Protein concentration was determinate by Coomassie Brilliant Blue.Malondialdehyde(MDA)

hydroxyl radical(OH ·)

total antioxidant capacity(T-ACO)

Superoxide dismutase(SOD) and glutathione reductase(GR) activity were tested.Results:1) Ginkgetin(add before ACR) can significantly raise the viability of the cells(P<0.05).2) Ginkgetin can degrade MDA and OH · of cells that treated by acrolein significantly(P<0.05).3) Ginkgetin raised Total antioxidant

GR and SOD activity of sertoli cells treated by acrolein significantly(P<0.05).Conclusion:Ginkgetin can protect sertoli cells of immature testes from ACR oxidative injury in vitro.