Objective:To estabhish rat model of epilepsy induced by the Lithium-repeated low-dose pilocarpine.Methods:A totol of 173SD male rats were divided into the normal control group

seizure-induced group and Chloral Hydrate group randomly.Lithium chloride was injected at a dose of 3 mEq/kg intraperitoneally(ip) 19h prior to the administration of pilocarpine.Rats were injected with pilocarpine

10mg/kg ip

every 30min

and this injection was repeated for 2~4 times.We then determined the rat activity

EEG

the percentage of rats exhibiting status epilepticus(SE)

the mortality rate

the proportion of rats developing spontaneous seizures

the latency for the first spontaneous motor seizure

and the frequency of the spontaneous recurrent seizures.Results:The lithium-repeated low-dose pilocarpine resulted in SE after 1~4 injections;the average period between seizure and injection was 16min and the success rate was 73%.The mean dose of pilocarpine needed to induce SE was 25.9mg/kg.When SE was interrupted after 90min

mortality rate was below 10%

Spontaneous seizure occurred 2.4~5.7 times a day averagely

and average latency to the first spontaneous seizure was approximately 40days.All seizure incidence was over level Ⅲ and most were level Ⅳ/Ⅴ;EEG was similar to that of human temporal epilepsy;seizure of rats in control group was level 0.Conclusion:Lithium-repeated low-dose pilocarpine induced rats model of epilepsy is characterized by easy building

with high successful rate and lower mortality of the animal.This model has mimic features of human status epilepticus and temporal epilepsy in seizure behavior and electroencephalographic findings.