小鼠胆总管结扎致肝纤维化模型的建立与评价

肖颜玉; 胥飚; 崔鲂; 张莉萍

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小鼠胆总管结扎致肝纤维化模型的建立与评价

论著 | 更新时间：2024-01-17

- 小鼠胆总管结扎致肝纤维化模型的建立与评价
- Establishment and evaluation of liver fibrosis model induced by bile duct ligation in mouse
- 重庆医科大学学报 2008年第4期页码：390-393
- 作者机构：
  
  1. 重庆医科大学附属第一医院检验科
  2. 重庆医科大学附属第一医院检验科,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然科学基金（30670965）;教育部重点课题（207099）
- DOI：
  中图分类号： R-332
- 纸质出版日期：2008，
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[1]肖颜玉,胥飚,崔鲂,张莉萍.小鼠胆总管结扎致肝纤维化模型的建立与评价[J].重庆医科大学学报,2008(04):390-393.

XIAO Yan-yu, et al. Establishment and evaluation of liver fibrosis model induced by bile duct ligation in mouse[J]. Journal of Chongqing Medical University, 2008,(4):390-393.
[1]肖颜玉,胥飚,崔鲂,张莉萍.小鼠胆总管结扎致肝纤维化模型的建立与评价[J].重庆医科大学学报,2008(04):390-393. DOI：

XIAO Yan-yu, et al. Establishment and evaluation of liver fibrosis model induced by bile duct ligation in mouse[J]. Journal of Chongqing Medical University, 2008,(4):390-393. DOI：

摘要

目的:建立小鼠胆总管结扎致肝纤维化模型

并对其进行初步评价。方法:随机给予小鼠胆总管结扎和假手术

进行血清学指标（AST、ALT、ALP、TBIL、DBIL）检测;HE染色和天狼猩红染色进行形态学观察;采用免疫组化染色分析α-SMA和CK-19表达;实时荧光定量PCR检测α-SMA、Collagen-1 mRNA的表达。结果:小鼠胆总管结扎后2周可见CK-19阳性的棕褐色细胞明显增生

模型组血清AST、ALT、ALP、TBIL、DBIL明显增高

α-SMA、Collagen-1表达明显增强。结论:成功构建小鼠胆总管结扎致肝纤维化模型。

Abstract

Objective:To establish and evaluate liver fibrosis model induced by bile duct ligation in mouse.Methods:The bile ducts of the mice were occluded by ligation.Serum AST

ALT

ALP

TBIL and DBIL were determined;the morphological observation was done by HE staining and Sirius red staining

expressions of α-SMA and CK-19 were analyzed by immunohistochemistry staining;mRNA expression of α-SMA and Collagen-1 in liver tissues were assayed by real-time PCR.A sham-operation group was used as a control.Results:Chocolate brown cells with positive expression of CK-19 and α-SMA significantly proliferated 2 weeks after bile duct ligation.Serum AST

ALT

ALP

TBIL and DBIL levels of model group were much higher than those of control group;mRNA expression of α-SMA and Collagen-1 in liver tissue by real-time PCR were increased significantly in model group.Conclusion:The mouse liver fibrosis model can be established successfully by bile duct ligation.

关键词

胆总管结扎肝纤维化动物模型

Keywords

Bile duct ligationliver fibrosisanimal model

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