siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖

沈娜; 张潜英; 余晓燕; 叶琳; 陈鸿雁

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siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖

论著 | 更新时间：2024-01-17

- siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖
- siRNA inhibits HPV16 E6 expression and cell proliferation in HNE-1 cell line
- 重庆医科大学学报 2008年第8期页码：927-930
- 作者机构：
  
  重庆医科大学附属第一医院耳鼻咽喉科
- 作者简介：
- 基金信息：
  
  重庆市卫生局资助项目（项目编号:07-1-021）
- DOI：
  中图分类号： R739.63
- 纸质出版日期：2008，
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[1]沈娜,张潜英,余晓燕,叶琳,陈鸿雁.siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖[J].重庆医科大学学报,2008(08):927-930.

SHEN Na, et al. siRNA inhibits HPV16 E6 expression and cell proliferation in HNE-1 cell line[J]. Journal of Chongqing Medical University, 2008,(8):927-930.
[1]沈娜,张潜英,余晓燕,叶琳,陈鸿雁.siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖[J].重庆医科大学学报,2008(08):927-930. DOI：

SHEN Na, et al. siRNA inhibits HPV16 E6 expression and cell proliferation in HNE-1 cell line[J]. Journal of Chongqing Medical University, 2008,(8):927-930. DOI：

摘要

目的:探讨小干扰RNA（Small interferirng RNA

siRNA）对鼻咽癌（Nasopharyngeal carcinoma

NPC）HNE-1细胞株HPV16E6基因表达的抑制作用

观察HPV16E6基因沉默对鼻咽癌细胞生长的影响。方法:体外合成4条针对HPV16E6基因的siRNA

通过脂质体转染导入鼻咽癌HNE-1细胞。用MTT检测转染后癌细胞的生长增殖情况

用半定量逆转录聚合酶链反应（Reverse transcription polymerase chain reaction

RT-PCR）检测转染后HPV16 E6 mRNA的表达水平

用免疫组化技术检测鼻咽癌细胞中HPV16 E6蛋白的表达水平。结果:导入有效siRNA后

MTT实验显示细胞的生长增殖受到明显抑制

抑制率可达32%;PT-PCR及免疫组化结果显示RNA干扰（RNA interfering

RNAi）可使HNE-1细胞中HPV16 E6 mRNA水平及蛋白表达水平下降。结论:siRNA可以有效抑制HNE-1细胞株中HPV16 E6的表达

降低鼻咽癌细胞的增殖能力。RNAi技术作为一种新的基因治疗技术在鼻咽癌治疗方面有着巨大的应用潜力。

Abstract

Objective:To evaluate the inhibitory effect of Small interferirng RNA(siRNA) on human papilloma vinus(HPV)16 E6 oncogenes in nasopharyngeal carcinoma(NPC) cell line HNE-1

and observe the effects of HPV16 E6 silencing on NPC cell growth.Methods:Four siRNA against the HPV16 E6 gene were designed and transfected into HNE-1 cell respectively using RNAi-Mate transfection reagent.MTT was used to investigate the cellular proliferation after the transfection.RT-PCR was used to detect the expression of HPV16 E6 and protein level of HPV16 E6 was measured by Immunohistochemical staining.Results:After the most effective siRNA was transfected into HNE-1cell

the result of MTT indicated that the cellular proliferation was restrained remarkably

and the highest inhibitory rate was 32%.Meanwhile

RT-PCR and immunohistochemical staining showed that there was a significant decrease in HPV16 E6 mRNA and protein level.Conclusion:These results demonstrated that siRNA against HPV16 E6 could effectively downregulate HPV16 E6 expression in HNE-1 cell line

and inhibit the cellular proliferation.RNAi has great potential in the treatment of nasopharyngeal carcinoma as a new gene therapy.

关键词

siRNAHPV16 E6RNA干扰鼻咽癌基因沉默逆转录聚合酶链反应

Keywords

siRNAHPV16 E6RNAinasopharyngeal carcinomaGene silenceReverse transcription polymerase chain reaction

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