Objective:To establish a HPLC method for measurement of serum concentration of sinomemine in rabbits and explore it’s pharmacokinetics. Methods:6 rabbits were intragastricly administrated sinomenine with the dosage of 45mg/kg

2 ml blood were collected at 0.25h、0.5h、1.0h、1.5h、2.0h、3.0h、4.0h、6.0h、8.0h、10.0h and 12.0h after oral administration.concentrations of drug were determined by HPLC

datas were calculated with 3P97 software and pharmacokinetics was explored. Results:T1/2(Ka) was(0.31±0.26)h

T1/2(Ke) was(3.15±0.82)h

Tmax was(1.05±0.53)h

Cmax was(15.09±4.15)μg/ml

AUC0→∞ was(84.27±13.02)(μg·h)/L

AUC0→T was(78.29±13.46)(μg·h)/L. Conclusion:absorption and elimination of Sinomenine were quickly and Tmax is short

concentration of drug is high in rabbits after intragastric administration.