Objective:To explore the effect of heterogeneous nuclear ribonucleoprotein K(hnRNP K)short hairpin RNA(shRNA)on K562 cells. Methods:hnRNP K shRNA retrovirus was packaged with pSUPER retro hnRNP K shRNA after its identification by enzyme digestion and sequencing analysis

then FCM

Wright staining

MTT assay

RT-PCR were used to analyze its effects on cell cycle and apoptosis

proliferation

hnRNP K and eukaryotic translation initiation factor-4E(eIF4E) mRNA of K562 cells respectively. Results:The sequence of the inserted fragment in pSUPER retro hnRNP K shRNA was correct. Compared with the control K562 cells

hnRNP K shRNA treated group showed significantly more cells at G2/M phase(P<0.05)

growth inhibition and more cells with ≥2 nucleus(P<0.05) as well as 55.22% and 55.27% decrease of hnRNP K and eIF4E mRNA respectively. Conclusion:hnRNP K interference blocks K562 cells at G2/M phase

inhibits cell growth

and down-regulates both eIF4E and hnRNP K mRNA expression

increases cells with≥2 nucleus

suggesting that it might play a role during cell division.