Objective:To Clone the cDNA of human ICAT

and express it in tumor cell

to investigate its anti-tumor effect.Method:the ICAT cDNA was amplified by RT-PCR

the ukaryotic expression plasmid pcDNA3.1-ICAT was constructed

MCF-7 and MDA-MB231 breast cancer cells were transfected by pcDNA3.1-ICAT using liposome and the cancer cell proliferation was estimated by MTT.Result:The product of RT-PCR was about 260bp

its sequence was the same as that reported

the transfection efficiency for MCF-7 and MDA-MB-23 cells was about 26%

and inhibiting efficiencies on the proliferation of the cancer cell transfected were 18.1% and 16.2% respectively.Conlusion:The cDNA of ICAT was cloned

and its expression in MCF-7 and MDA-MB-23 cells may inhibit the proliferation of these cancer cells.