大蒜素联合顺铂对大鼠膀胱肿瘤生长抑制和诱导凋亡的实验研究

周生财; 唐伟

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大蒜素联合顺铂对大鼠膀胱肿瘤生长抑制和诱导凋亡的实验研究

论著_基础研究 | 更新时间：2024-01-17

- 大蒜素联合顺铂对大鼠膀胱肿瘤生长抑制和诱导凋亡的实验研究
- Research allicin allied with DDP on growth inhibition and induction apoptosis of rat bladder tumor
- 重庆医科大学学报 2008年第1期页码：45-48
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学附属第一医院泌尿外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R737.14
- 纸质出版日期：2008，
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[1]周生财,唐伟.大蒜素联合顺铂对大鼠膀胱肿瘤生长抑制和诱导凋亡的实验研究[J].重庆医科大学学报,2008(01):45-48.

ZHOU Sheng-cai, et al. Research allicin allied with DDP on growth inhibition and induction apoptosis of rat bladder tumor[J]. Journal of Chongqing Medical University, 2008,(1):45-48.
[1]周生财,唐伟.大蒜素联合顺铂对大鼠膀胱肿瘤生长抑制和诱导凋亡的实验研究[J].重庆医科大学学报,2008(01):45-48. DOI：

ZHOU Sheng-cai, et al. Research allicin allied with DDP on growth inhibition and induction apoptosis of rat bladder tumor[J]. Journal of Chongqing Medical University, 2008,(1):45-48. DOI：

摘要

目的:评价大蒜素联合顺铂治疗SD大鼠膀胱肿瘤的效果

探讨其作用机制。方法:膀胱灌注N-甲基亚硝基脲（MNU）诱导建立SD大鼠膀胱肿瘤模型

于第14周对5组荷瘤大鼠分别腹腔注射生理盐水

顺铂

不同浓度大蒜素

顺铂+大蒜素。每次注射0.2ml

隔日1次

共7次

其间观察实验动物生存状态。全部注射完毕后处死大鼠

称取膀胱总重量

对肿瘤组织进行常规石蜡切片

HE染色

光镜观察

TUNEL法检测肿瘤细胞凋亡情况。结果:生理盐水组、不同浓度大蒜素组、顺铂+大蒜素组的大鼠体重均随鼠龄的增长而增加

而顺铂组大鼠在停药后随鼠龄的增长体重明显降低

与其他各组比较

差异显著。顺铂+大蒜素组大鼠膀胱的总重量显著小于其他各组。TUNEL法原位检测发现各组均出现不同程度的肿瘤细胞凋亡

大蒜素+顺铂组尤为明显。结论:大蒜素联合顺铂可共同诱导细胞凋亡发挥协同效应

抑制膀胱肿瘤的生长

并且能改善大鼠的生存状态。

Abstract

Objective:To evaluate the validity of allicin allied with DDP on bearing tumor SD rats

explore the mechanisms of function.Methods:set up bladder cancer models by irrigating MNU into SD rat bladder

examine the incidence of cancer.At the end of 14th weeks

five groups were accepted peritoneal injection with NS

allicin

DDP

DDP + allicin respectively.one time every other day

every rat was injected 0.2ml different drugs every time

7 times in all

observe experimental animals life quality at the same time.After all rats were killed

bladders were weighed.Tumor tissues were embeded in paraffin

each sample was cut into 3 pieces for HE staining

and observed by light microscope.TUNEL method was applied to examine apoptosis of tumor cells.Results:in the DDP group

a marked decrease was shown in the appearance of weight when compared with the other groups.Apoptosis of bladder tumors occurred in all groups

especially in allicin allied with DDP groups.Conclusion:Allicin allied with DDP can inhibite bladder tumor growth by inducing apoptosis in common

and improve life quality.

关键词

大蒜素顺铂膀胱肿瘤凋亡

Keywords

AllicinDDPBladder tumorapoptosis

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