Objective:To establish rat models with Alzheimer’s disease(AD)induced by β-Amyloid1-40

and to observe the effects of Fastigial nucleus stimulation(FNS)on spatial memory and learning ability and the expression of bcl-2 and Bax of hippocampus area in the rats.Methods:Aβ1-40 was microinjected into CA1 subfield in the right hippocampus of rats.Cerebellar dentate nucleus and fastigial nucleus had been stimulated separately before the model was made.The spatial memory and learing ability of the rats were evaluated by the Morris water maze procedure in 28 days after the injection.The expression of bcl-2 and Bax were determined by immunohistochemistry method.Results:The escape latency of the place navigation in the AD group was singnificiantly longer than the sham injection group(P<0.05).Aβ1-40 could reduce the optical density of positive cells of bcl-2(P<0.05)and increase the optical density of positivecells of Bax(P<0.05)of hippocampus area.The AD rats receiving FNS showed significiant shorter escape latency than AD rat(sP<0.05).FNS could increase the optical density of positive cells of bcl-2(P<0.05)and reduce the optical density of positive cells of Bax(P<0.05)of hippocampus area.Dentate nucleus stimulation showed no such effect.Conclusion:FNS can partlly improve the spatial learning and memory dysfunction of AD rat.It can inhibit apoptosie and has neuroprotective effect of hippocampal cells by inhibiting the expression of Bax and promoting the expression of bcl-2 of hippocampus area.