当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究

宋姝丹; 王建伟; 王亚平; 姜蓉

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当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究

论著_基础研究 | 更新时间：2024-01-17

- 当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究
- Experimental study on the effect of angelica polysaccharide on inhibition of proliferation and induced differentiation towards erythroid cell of human erythroleukemia cell line(K562)
- 重庆医科大学学报 2008年第1期页码：1-5
- 作者机构：
  
  1. 重庆医科大学基础医学院组织胚胎学教研室
  2. 重庆医科大学基础医学院组织胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（30572429）
- DOI：
  中图分类号： R285
- 纸质出版日期：2008，
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[1]宋姝丹,王建伟,王亚平,姜蓉.当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究[J].重庆医科大学学报,2008(01):1-5+12.

SONG Shu-dan, et al. Experimental study on the effect of angelica polysaccharide on inhibition of proliferation and induced differentiation towards erythroid cell of human erythroleukemia cell line(K562)[J]. Journal of Chongqing Medical University, 2008,(1):1-5.
[1]宋姝丹,王建伟,王亚平,姜蓉.当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究[J].重庆医科大学学报,2008(01):1-5+12. DOI：

SONG Shu-dan, et al. Experimental study on the effect of angelica polysaccharide on inhibition of proliferation and induced differentiation towards erythroid cell of human erythroleukemia cell line(K562)[J]. Journal of Chongqing Medical University, 2008,(1):1-5. DOI：

摘要

目的:研究当归多糖（Angelica polysaccharide

APS）对人红白血病细胞株（K562）增殖抑制及定向红系细胞分化的影响。方法:采用细胞体外培养技术、MTT法、台盼蓝拒染法与流式细胞术检测APS对K562细胞增殖的影响。光镜观察细胞形态变化;联苯胺染色与分光光度法检测经APS诱导后K562细胞向红系分化的特征和血红蛋白含量的变化。结果:APS对K562细胞的增殖有明显的抑制作用

抑制强度与APS的剂量和作用时间密切相关;经APS诱导后K562细胞的联苯胺染色阳性率增高

合成血红蛋白的量提高。结论:APS在体外对K562细胞有明显的增殖抑制及诱导其向红系细胞分化的作用。

Abstract

Objective:To investigate the effect of Angelica polysaccharide on inhibition of proliferation and induced erythrocytic differentiation of human erythroleukemia cell line(K562).Methods:Techniques of cell culture were used in this experiment.The effect of APS on proliferation of K562 was examined by MTT、trypan blue staining and flow cytometry.The change of cell form was observed by Wright’s stain.The effect of APS induced erythrocytic differentiation of K562 was detected by cytochemistry and spectrophotometric method.Results:APS can significantly inhibit the proliferation of K562 ex vivo

and the inhibitory extent is paralleled to the concentration and the acting time of APS;APS promoted the expression of Hb.Conclusion:APS can significantly inhibit the proliferation and induce the differentiation towards erythroid cell of K562 ex vivo.

关键词

当归多糖K562抑制分化红系细胞

Keywords

APSK562Inhibition of proliferationdifferentiationErythroid cell

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