YIN Zhi-kang, et al. Anoxia-reoxygenation up-regulates transcription of TGFβ1 gene in human umbilical veins endothelial cell line[J]. Journal of Chongqing Medical University, 2008,(6):694-696.
YIN Zhi-kang, et al. Anoxia-reoxygenation up-regulates transcription of TGFβ1 gene in human umbilical veins endothelial cell line[J]. Journal of Chongqing Medical University, 2008,(6):694-696.DOI:
Objective:To investigate the influence of anoxia-reoxygenation on the transcription of TGFβ1 gene in Human umbilical veins endothelial cell line(ECV304). Methods:Anoxia-reoxygenation model of Human umbilical veins endothelial cell line was established. TGFβ1 mRNA was half-quantitatively analyzed by a modified RT-PCR. Results:The transcription of TGFβ1 mRNA was up-regulated significantly in anoxia-reoxygenation group compared with the control group. Conclusion: anoxia-reoxygenation may up-regulate the transcription of TGFβ1 gene in human umbilical veins endothelial cell line.