Objective:To investigate the effect of advanced glycosylation end-products(AGEs) on the expression of peroxisome proliferator-activated receptor-γ(PPARγ) in THP-1 macrophages and explore the function of AGEs in atherosclerosis.Methods: Differentiation of THP-1 cell was induced by PMA.The THP-1 macrophages were exposed to AGE-modified bovine serum albumin(AGE-BSA) in different concentration or the same concentration for different time.Expression of PPARγ mRNA and protein in THP-1 macrophages was measured by RT-PCR and immunocytochemical method.Results:RT-PCR showed that expression of PPARγ mRNA was 1.235±0.044

0.752±0.055

0.494±0.026

0.277±0.025 in 50

100

200 and 400μg/ml AGE-BSA groups.PPARγ mRNA expression in THP-1 macrophages following 200μg/ml AGE-BSA for 12

24

36

48h was 1.260±0.043

0.467±0.033

0.360±0.012.Western-Blot showed that after exposure of THP-1 macrophages to 50

100

200and 400mg/L AGE-BSA

the average integrated optical density values of PPARγ protein expression were 36.460±0.625

24.561±0.636

19.326±0.803

12.715±0.752 respetively

significantly lower than that in BSA group(P<0.05)

the average integrated optical density values of PPARγ protein expression in THP-1macrophages following 200mg/L AGE-BSA for 12

24

36

48h were 32.502±0.739

22.234±0.835

16.568±0.683

11.537±0.547

lower than that in 0h group(P<0.05).Conclusion:AGE-BSA could decrease the expression levels of mRNA and proteins for PPARγ in THP-1 macrophages in a dose-and time-dependent manner

which might be partly involved in atherogenesis in diabetic patients.