objective:To identify the function of NRP-1 in progression and angiogenesis of laryngocarcinoma cell line Hep-2 in vitro.methods:MTT

the cell migrate

attack and vascular formation experiments in vitro was used to evaluate the effect of NRP-1 antigen on the migrate

attack and angiogenesis capability of laryngocarcinoma cell line Hep-2.Results:The proliferation inhibition ratio of Hep-2 cells was risen up as the NRP-1 antigen’s concentration was increaced(r=0.624

P<0.01).The number of cells migrated in the group with NRP-1 antigen was fewer than that in the control group(P<0.01).After Hep-2 cells were treated with NRP-1 antigen for 48 hours

compared with the control group

the adhesion

invasion ability was significantly weakened(P<0.01)and induction of vessel lumina formation on matrigel was much less in vitro(P<0.01).Conclustion:The NRP-1 antigen suppressed the Hep-2 cell line’s proliferation

adhesion

migration

invasion

and angiogenesis in vitro

confirmed that NRP-1 not only played an impotant role in vascular endothelial cell angiogenesis

but also had remarkable influence on Hep-2 cells.It suggested that NRP-1 may plays an impotant role in the laryngocarcinoma’s tumorigenesis and developing process

inhibit the expression of NRP-1 may be a new stratage in the treatment of laryngocarcinoma