柳氮磺胺吡啶对HSC-T6和L02细胞增殖和凋亡的初步观察

朱慧利; 沈薇; 陈忠勇

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柳氮磺胺吡啶对HSC-T6和L02细胞增殖和凋亡的初步观察

论著 | 更新时间：2024-01-17

- 柳氮磺胺吡啶对HSC-T6和L02细胞增殖和凋亡的初步观察
- Preliminary observation on proliferation and apoptosis of HSC-T6 and L02 induced by sulfasalazine
- 重庆医科大学学报 2008年第9期页码：1054-1057
- 作者机构：
  
  1. 重庆医科大学附属第二医院消化内科
  2. 凉山州第二人民医院肝胆外科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R96
- 纸质出版日期：2008，
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[1]朱慧利,沈薇,陈忠勇.柳氮磺胺吡啶对HSC-T6和L02细胞增殖和凋亡的初步观察[J].重庆医科大学学报,2008(09):1054-1057.

ZHU Hui-li, et al. Preliminary observation on proliferation and apoptosis of HSC-T6 and L02 induced by sulfasalazine[J]. Journal of Chongqing Medical University, 2008,(9):1054-1057.
[1]朱慧利,沈薇,陈忠勇.柳氮磺胺吡啶对HSC-T6和L02细胞增殖和凋亡的初步观察[J].重庆医科大学学报,2008(09):1054-1057. DOI：

ZHU Hui-li, et al. Preliminary observation on proliferation and apoptosis of HSC-T6 and L02 induced by sulfasalazine[J]. Journal of Chongqing Medical University, 2008,(9):1054-1057. DOI：

摘要

目的:探讨柳氮磺胺吡啶（Sulfasalazine

SASP）对正常肝细胞和肝星状细胞（Hepatic stellate cell

HSC）增殖和凋亡的影响。方法:以正常人肝细胞株（L02细胞）和大鼠肝星状细胞株（HSC-T6细胞）为研究靶细胞

用CCK-8比色法检测细胞增殖;流式细胞术Annexin V-FITC/PI双染检测HSC-T6细胞凋亡率;AO/EB染色法观察细胞凋亡形态。结果:同对照组比较

一定浓度范围的SASP对HSC的增殖具有显著的抑制作用

呈现时间和剂量效应。膜联蛋白（Annexin V）/碘化丙啶（PI）双染显示SASP诱导HSC-T6凋亡也呈现时间和剂量效应;AO/EB染色出现凋亡细胞的特征性改变。而SASP干预L02后

与对照组相比

增殖和凋亡率均无统计学意义

AO/EB染色后

未出现凋亡细胞。结论:同等干预浓度的SASP能够选择性的抑制HSC增殖

诱导其凋亡。

Abstract

Objective:To investigate the effect of sulfasalazine on proliferation and apoptosis of HSC-T6 and L02 in culture. Methods:HSC-T6 and L02 were incubated with different concentration of sulfasalazine.Cell proliferation was assessed by CCK-8 assay

cell apoptosis was analyzed by annexin Ⅴ FITC/PI flow cytometry;and apoptotic morphology was examined by vital staining of acridine orange/ethidium bromide(AO/EB). Results:Compared with the control group

the proliferation of HSC-T6 was significantly inhibited by sulfasalazine in a time and dose-dependant manner

when the concentration of sulfasalazine in the medium reached a certain level range. The HSC-T6 apoptotic induced by sulfasalazine was confirmed by AO/EB and annexin Ⅴ FITC/PI.compared with the control group

the proliferation and apoptosis rate of L02 treated by group had no statistical significance

and AO/EB staining showed no apoptosis. Conclusion:Sulfasazinea at the same concentration can specifically inhibit the proliferation of HSC and induce HSC apoptosis.

关键词

HSC-T6L02增殖凋亡柳氮磺胺吡啶

Keywords

HSC-T6L02proliferationapoptosisSulfasalazine(SASP)

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