Objective:To evaluate the activity of nuclear factor kappaB(NF-κB)and the gene expression of high mobility group box 1(HMGB1)in lung injury induced by intestinal ischemia-reperfusion(IIR)

and to investigate the protective effect of polydatin(PD)in this lung injury.Methods:Fifty-four male Sprague-Dawley rats weighing 200~250 g were randomly divided into control group

IIR group(45 min intestinal ischemia with 0.5 h or 2 h or 6 h reperfusion)and PD group [IIR with PD(10 mg/kg)treatment].The level of pulmonary myeloperoxidase(MPO)was detected by colorimetric method

the expressions of NF-κB and ICAM-1 by immuneochemistry and that of pulmonary HMGB1mRNA by RT-PCR respectively.Results:Lung injury induced by IIR was characterized by pathological damage of edema

hemorrhage and inflammatory cell infiltration.Compared with control group

pulmonary W/D and MPO increased significantly(P<0.05).Positive expressions of NF-κB p65 and ICAM-1 protein

and HMGB1mRNA(2 h、6 h)also increased significantly(P<0.05).After administration of PD

the levels of pulmonary indices were improved significantly(P<0.05)

pulmonary damage alleviated compared with IIR group(P<0.05)

and the expresion of NF-κB became weak.Conclusion:Injection of polydatin before reperfusion could alleviated lung injury induced by intestinal ischemia-reperfusion in rats in vivo

which is possibly through inhibiting pulmonary NF-κB activation and attenuating the expression of HMGB1mRNA during lung injury in rats.