Objective:To investigate the changes of myocardial energy production in septic mice after a cecal ligation and puncture

and to observe the intervention effects of Shenfu injection.Methods:Mice models of abdominal infection were established by a cecal ligation and puncture(CLP).Eighty-three mice were randomly divided into three groups:CLP group(infection group)

CLP and treated with Shenfu injection group(treatment group)and pseudooperation control group.The morphology and membrane phospholipid of myocardial mitochondria were studied by using transmission electronmicroscope morphometry method.The activities of cytochrome oxidase(CCO)and succinate dehydrogenase(SDH)of myocardial mitochondria were also detected by enzyme cytochemical method.Reversed phase high-performance liquid chromatography(RP-HPLC)was used to analyze contents of myocardial ATP

ADP and AMP.Results:As compared to control group

a large number of mitochondria were damaged in infection group

the apparent mitochondrial membrane phospholipid deletion and localization change were observed

the activities of CCO and SDH in mitochondria declined obviously

and the contents of ATP

ADP as well as AMP declined notably.All of these were obviously improved in treatment group.Conclusion:The myocardial mitochondrial structure destroyed and its functions declined apparently in septic mice.Shenfu injection could protect the myocardial mitochondrial structure and function effectively

improve the myocardial energy metabolism in septic mice also.