高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸

邓文平; 王文娟; 邵勇; 邓丹; 丁敏

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高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸

技术方法 | 更新时间：2024-01-17

- 高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸
- Simultaneous determination of serum tauro-conjugated bile acids in intrahepatic cholestasis of pregnancy patients by high performance liquid chromatography-tendem mass spectrometry
- 重庆医科大学学报 2009年34卷第6期页码：732-734
- 作者机构：
  
  1. 重庆医科大学医学检验系临床检验诊断学教育部重点实验室
  2. 重庆医科大学附属第一医院妇产科
  3. 重庆医科大学公共卫生学院卫生统计教研室
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（20475069）
- DOI：
  中图分类号： R714.255
- 纸质出版日期：2009，
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[1]邓文平,王文娟,邵勇,邓丹,丁敏.高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸[J].重庆医科大学学报,2009,34(06):732-734.

DENG Wen-ping, et al. Simultaneous determination of serum tauro-conjugated bile acids in intrahepatic cholestasis of pregnancy patients by high performance liquid chromatography-tendem mass spectrometry[J]. Journal of Chongqing Medical University, 2009,34(6):732-734.
[1]邓文平,王文娟,邵勇,邓丹,丁敏.高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸[J].重庆医科大学学报,2009,34(06):732-734. DOI：

DENG Wen-ping, et al. Simultaneous determination of serum tauro-conjugated bile acids in intrahepatic cholestasis of pregnancy patients by high performance liquid chromatography-tendem mass spectrometry[J]. Journal of Chongqing Medical University, 2009,34(6):732-734. DOI：

摘要

目的:研究妊娠肝内胆汁淤积症（Intrahepatic cholestasis of pregnancy

ICP）患者血清中牛磺结合型胆汁酸的变化。方法:收集ICP及ICP伴糖尿病、ICP伴病毒性肝炎患者和正常孕妇血清

经固相萃取

采用高效液相色谱-串联质谱（Highperfor-mance liquid chromatography-tendem mass spectrometry

HPLC-MS/MS）法测定牛磺结合型胆汁酸的含量。结果:ICP及ICP伴糖尿病、ICP伴病毒性肝炎患者血清中牛磺胆酸（Taurocholicacid

TCA）、牛磺鹅脱氧胆酸（Taurochenodeoxycholic acid

TCDCA）、牛磺脱氧胆酸（Taurodeoxycholic acid

TDCA）分别比正常孕妇有10倍、5倍和3倍以上的显著性增高（P<0.01）。ICP及ICP伴糖尿病、ICP伴病毒性肝炎三组间TCA和TCDCA的差异均有显著性（P<0.05）。结论:ICP患者有特征的血清牛磺结合型胆汁酸变化。

Abstract

Objective:To investigate the individual serum tauro-conjugated bile acid concentration in patients with Intrahepatic Cholestasis of Pregnancy(ICP) by high performance liquid chromatography-tendem mass spectro-metry(HPLC-MS/MS).Methods:Bile acids were extracted from serum samples of ICP

ICP with Diabetes Mellitus

ICP with Virus Hepatitis patients and normal pregnant women.Serum tauro-conjugated bile acids were measured by HPLC-MS/MS.Results:The serum levels of taurocholic acid(TCA)

taurochenode-oxycholic acid(TCDCA) and taurodeoxycholic acid(TDCA) in the three groups of ICP

ICP with Diabetes Mellitus

and ICP with Virus Hepatitis were significantly higher than those of the control group(P<0.01)

and the differences and were 15-fold

5-fold

3-fold

respectively.The serum levels of TCA and TCDCA were statistically different among each two of patients of ICP

ICP with Diabetes Mellitus

ICP with Virus Hepatitis respectively(P<0.05).Conclusion:The concentrations of individual tauro-conjugated bile acids have specific features in ICP patients.

关键词

胆汁酸血清妊娠肝内胆汁淤积症高效液相色谱串联质谱

Keywords

Bile acidsserumICPHPLCMS/MS

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