CIK细胞对裸鼠人肝癌转移预防和治疗

陈国林; 胡怀东; 袁迪; 李用国; 任红; 马英骥

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CIK细胞对裸鼠人肝癌转移预防和治疗

论著 | 更新时间：2024-01-17

- CIK细胞对裸鼠人肝癌转移预防和治疗
- Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)
- 重庆医科大学学报 2008年第11期页码：1338-1340
- 作者机构：
  
  1. 哈尔滨医科大学附属第一医院感染科
  2. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R735.7
- 纸质出版日期：2008，
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[1]陈国林,胡怀东,袁迪,李用国,任红,马英骥.CIK细胞对裸鼠人肝癌转移预防和治疗[J].重庆医科大学学报,2008(11):1338-1340.

CHEN Guo-lin, et al. Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)[J]. Journal of Chongqing Medical University, 2008,(11):1338-1340.
[1]陈国林,胡怀东,袁迪,李用国,任红,马英骥.CIK细胞对裸鼠人肝癌转移预防和治疗[J].重庆医科大学学报,2008(11):1338-1340. DOI：

CHEN Guo-lin, et al. Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20)[J]. Journal of Chongqing Medical University, 2008,(11):1338-1340. DOI：

摘要

目的:探讨人细胞因子杀伤（Cytokine-inducedkiller

CIK）细胞对裸鼠人肝癌转移模型（LCI-D20）治疗作用。方法:用淋巴细胞分离液分离外周血单个核细胞

加入细胞因子rhIFN-γ、人CD3mAb和rhIL-2

诱导生成CIK细胞

经腹腔注射

同时应用PBS作为对照

研究其对LCI-D20肝癌治疗和预防转移作用。结果:CIK组和对照组肝癌肿块重量、血AFP含量、肝癌肝内转移率和生存期依次分别为（1.125±0.363）g和（2.682±0.526）g;（48.6±5.2）μg/L和（82.2±7.2）μg/L;16.7%和58.3%;（73.3±7.03）d和（52.3±5.24）d

与对照组比较均P<0.01。结论:CIK细胞可以预防LCI-D20模型肝癌发生转移

延长动物生存时间。

Abstract

Objective:To investigate the cure effect of human cytokine-induced killer (CIK) cells on the model of human hepatocellular carcinoma in nude mice. Methods:Peripheral blood mononuclear cells(PBMC)were obtained from healthy volun- teers by Ficoll gradient centrifugation and CIK cells were induced by culturing PBMC in the completed medium containing rhIFN-γ

rhIL-2 and mAb against CD3.By intraperitoneal injection of CIK cells in LCI-D20

the effect of prevention and therapy to HCC in LCI-D20 were assessed. Results:The weight of tumor in the CIK cells group and negative group was (1.125±0.363)gram and(2.682±0.526)gram respectively(t=6.06

P<0.01). The amount of AFP in blood of the group of CIK cells was(48.6±5.2)μg/L as the amount in the negative group was (82.2±7.2)μg/L(t=22.07

P<0.01). The metastasis rate in liver was 16.7% and 58.3% between two groups(χ2=4.44

P<0.01). The survival time of model in the therapy group were(73.3±7.03) days in the CIK group as compared with (52.3±5.24)days in negative group

(t=17.54

P<0.01). Conclusion:CIK cells may prevent metastasis in the model of LCI-D20 and prolong the survival time.

关键词

细胞因子杀伤细胞癌肝细胞

Keywords

Cytokine-induced killer cellscarcinomaHepatocellular

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