Objective:To observe the effect of GSK-3β on the cell cycle and metastasis of human gastric carcinoma cell.Methods:Influencing the GSK-3β activity of human gastric carcinoma cell line SCG-7901 by LY294002 with or with not LiCl treatment.The expression of total GSK-3β and p-GSK-3βser9 were detected in protein level by western blotting

cell proliferation and cell cycle distribution were tested by MTT and FCM respectively

the expression of E-cadherin and β-catenin were measured with S-P immunohistochemistry and the cell-ground substance adhesion was tested by cell adherence assay.Results:The expression of total GSK-3β was constant and the protein level of p-GSK-3β ser9 was down regulated after exposured to LY294002 by 72 h

the cell proliferation was inhibited

cell cycle was arrest at G0/G1 phase

the E-cadherin level was positively regulated

the expression of β-catenin and the adhesion rate were decreased.The effect on the proliferation、cell cycle、the expression of E-cadherin and β-catenin and the adhesion by the LY294002 were partially abrogated by lithium chloride.Conclusion:GSK-3βcould negatively regulate the proliferation of human gastric carcinoma cell by leading cells to G1-S arrest and inhibit its metastasis by positively regulating the expression of E-cadherin.