Objective:To develop a HPLC method with ultraviolet detection to determine nine types of conjugated bile acids in human bile.Methods:Bile acids in bile juice were extracted using Sep-Pak C18 cartridges.The analytes were eluted using methanol/phosphate buffer(60 μmol/L

pH3.3)(66:34

v/v)as mobile phase on a Waters Nova C18 column.The flow rate was 0.8 ml/min.The column was kept at 25℃.The eluate was monitored by a ultraviolet detection at 197nm.Results:The nine types of conjugated bile acids were completely analyzed in twenty minitus by this method.The assay was linear in the range 8~100 0 μmol/L

with the correlation coeffients for linear regression from 0.9991 to 0.999 9.The detection limits ranged from 1.25 to 3.12 μmol/L.The within-day precision was below 8.20%

and the between-day precision was below 17.11%.The recoveries were 88.23%~109.75%.Conclusion:This method is accurate

sensitive

rapid and suitable for the rountine clinical practice and research practice.