斑蝥酸钠抑制人肝癌HepG<sub>2</sub>细胞增殖及其机制的研究

高伟; 郑军

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斑蝥酸钠抑制人肝癌HepG₂细胞增殖及其机制的研究

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- 斑蝥酸钠抑制人肝癌HepG₂细胞增殖及其机制的研究
- Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms
- 重庆医科大学学报 2008年第11期页码：1356-1358
- 作者机构：
  
  重庆医科大学附属第一医院普通外科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R735.7
- 纸质出版日期：2008，
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[1]高伟,郑军.斑蝥酸钠抑制人肝癌HepG_2细胞增殖及其机制的研究[J].重庆医科大学学报,2008(11):1356-1358.

GAO Wei, et al. Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms[J]. Journal of Chongqing Medical University, 2008,(11):1356-1358.
[1]高伟,郑军.斑蝥酸钠抑制人肝癌HepG_2细胞增殖及其机制的研究[J].重庆医科大学学报,2008(11):1356-1358. DOI：

GAO Wei, et al. Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms[J]. Journal of Chongqing Medical University, 2008,(11):1356-1358. DOI：

摘要

目的:探讨斑蝥酸钠对肝癌HepG2细胞增殖的作用及其机制。方法:肝癌HepG2细胞在不同浓度斑蝥酸钠干预后分别采用MTT法、流式细胞术、免疫细胞化学法、比色法等检测细胞增殖抑制率、细胞周期变化、Survivin蛋白表达和Caspase-3活性。结果:斑蝥素能明显抑制肝癌HepG2细胞增殖。药物干预后

细胞出现G2/M期阻滞

Survivin蛋白表达抑制

Caspase-3活性增强。结论:斑蝥酸钠可能通过其G2/M期特异性阻滞

下调Survivin表达和激活Caspase-3来抑制HepG2细胞增殖。

Abstract

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate

The in- hibiton of cell proliferation

cell cycle distribution

expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay

flow cytometry

immunocytochemistry

and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24

72 h after intervention were 2.41±0.48

0.72±0.08

and 0.39±0.04 ug/ml

The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle

enhancement of Caspase-3

and inhibition of Survivin.

关键词

斑蝥酸钠肝癌细胞SurvivinCaspase-3

Keywords

Disodium cantharidinateHepatic carcinoma cellSurvivinCaspase-3

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斑蝥酸钠抑制人肝癌HepG2细胞增殖及其机制的研究

Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and its molecular mechanisms

DOI：

摘要

Abstract

关键词

Keywords

references

斑蝥酸钠抑制人肝癌HepG₂细胞增殖及其机制的研究

Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms