改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素

刘小玲; 颜伟伟; 王进; 张彦; 易钢; 何於娟

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改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素

技术方法 | 更新时间：2024-01-17

- 改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素
- Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation
- 重庆医科大学学报 2009年34卷第2期页码：203-205
- 作者机构：
  
  重庆医科大学临床检验诊断学教育部重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（No:30171150）
- DOI：
  中图分类号： R284
- 纸质出版日期：2009，
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[1]刘小玲,颜伟伟,王进,张彦,易钢,何於娟.改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素[J].重庆医科大学学报,2009,34(02):203-205.

LIU Xiao-ling, et al. Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation[J]. Journal of Chongqing Medical University, 2009,34(2):203-205.
[1]刘小玲,颜伟伟,王进,张彦,易钢,何於娟.改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素[J].重庆医科大学学报,2009,34(02):203-205. DOI：

LIU Xiao-ling, et al. Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation[J]. Journal of Chongqing Medical University, 2009,34(2):203-205. DOI：

摘要

目的:建立改良的快速分离、检测川楝树皮提取物中川楝素（Toosendanin

TSN）的超声波萃取-高效液相色谱法。方法:采用超声波乙醇提取-氯仿萃取制备川楝树皮提取物

高效液相色谱法（High performance liquid chromatography

HPLC）分析提取物中的TSN含量

以乙腈-水（45∶55）为流动相

检测波长215nm

流速1ml/min

柱温:25℃

色谱柱为XDBC18柱（4.6×250mm

5μm）。结果:TSN在4.64～88.2μg/ml浓度范围内有良好的线性关系（r=0.9996）

测定方法平均加标回收率为100.83%

精密度试验RSD为1.42%

稳定性试验RSD为2.26%。提取物中TSN经HPLC分离提纯后纯度达96.32%。结论:本方法简便、快速、准确

所得结果稳定、重复性好

可作为实验室少量制备和鉴定TSN纯品的方法。

Abstract

Objective:To establish an improved transonic extraction-high performance liquid chromatography(HPLC)method for separating and detecting toosendanin(TSN)in Melia toosendanin extractive.Methods:Transonic alcohol-chloroform extraction method was used to extract TSN from the bark of Melia toosendanin sieb.et zuccc.The content determination of TSN was performed on an Agilent XDB C18 Column(4.6×250 mm

5μm).Elution was performed using 45%acetonitrile-water solution as the mobile phase at a1flow rate of 1ml/min.Detection was performed at 215 nm wavelength.Results:The linear regression equation for peak area-TSN concentration(4.64～88.2μg/ml)was:A=7.616 2C-2.567 7

r=0.999 6;mean recovery of standard substance was 100.83%;RSD of precision test was 1.42%;RSD of stability test was 2.26%.Conclusion:The method is simple

rapid

stable and roducible

which can be used as a method for preparation and identification TSN of Melia toosendanin extractive in laboratory.

关键词

川楝素超声波萃取高效液相色谱法

Keywords

ToosendaninTransonic extractionHigh performance liquid chromatograph(yHPLC)

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