Objective:To explore the possible molecular mechanism of inhibiting the proliferation of lung cancer cells by studing curcumin regulating neucleoprotein β-catenin

the key factor of Wnt signaling pathway.Methods:Non-toxic different doses of curcumin affected human lung adenocarcinoma cell A549 for 48 hours.Inhibitory effect of curcumin on proliferation of A549 cells was evaluated by MTT assay.The correclation of doses and inhibition ratio was analyzed statistically.The neucleoprotein levels of β-catenin was analyzed by Western blot.The expression level of c-myc was analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The exchange of cell cycle was examined by flow cytometry(FCM).Results:Curcumin inhibited the proliferation of A549 in a concentration-dependent manne(rP<0.05)

and there was obvious linear correlation between drug concentrations and inhibition ratio(r=0.933 3).The expresson of nucleoprotein β-catenin and oncogene c-myc were all down-regulated in A549 cells(P<0.05)after treatment with curcumin.Curcumin prevented A549 cells from G1 to S phase period

and raised the percentage of G0/G1 phase cells(P<0.05).Conclusion:Curcumin could prevent β-catenin protein of A549 cells into the nucleus from the cytoplasm

block Wnt signal transduction pathway and inhibit the expression of downstream target genes c-myc.Thereby

curcimin prevents lung cancer cells from G1 phase to S phase

and effectively inhibit the proliferation of A549 cells.