pIL-12质粒DNA对细粒棘球蚴感染小鼠脾细胞凋亡的影响

张长碧; 李文桂; 朱佑明; 王鸿

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pIL-12质粒DNA对细粒棘球蚴感染小鼠脾细胞凋亡的影响

基础研究 | 更新时间：2024-01-17

- pIL-12质粒DNA对细粒棘球蚴感染小鼠脾细胞凋亡的影响
- Effects of pIL-12 plasmid DNA on apoptosis of splenocytes in mice infected with hydatid cyst of Echinococcus granulosus
- 重庆医科大学学报 2009年34卷第2期页码：142-145
- 作者机构：
  
  重庆医科大学附属第一医院传染病寄生虫病研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30801052,30671835,30500423和30200239）
- DOI：
  中图分类号： R392
- 纸质出版日期：2009，
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[1]张长碧,李文桂,朱佑明,王鸿.pIL-12质粒DNA对细粒棘球蚴感染小鼠脾细胞凋亡的影响[J].重庆医科大学学报,2009,34(02):142-145.

ZHANG Chang-bi, et al. Effects of pIL-12 plasmid DNA on apoptosis of splenocytes in mice infected with hydatid cyst of Echinococcus granulosus[J]. Journal of Chongqing Medical University, 2009,34(2):142-145.
[1]张长碧,李文桂,朱佑明,王鸿.pIL-12质粒DNA对细粒棘球蚴感染小鼠脾细胞凋亡的影响[J].重庆医科大学学报,2009,34(02):142-145. DOI：

ZHANG Chang-bi, et al. Effects of pIL-12 plasmid DNA on apoptosis of splenocytes in mice infected with hydatid cyst of Echinococcus granulosus[J]. Journal of Chongqing Medical University, 2009,34(2):142-145. DOI：

摘要

目的:探讨pIL-12质粒DNA免疫对细粒棘球蚴感染小鼠脾细胞凋亡的影响。方法:将pIL-12质粒DNA肌肉注射免疫BALB/C鼠

免疫后8周用Eg原头节进行攻击感染

感染后18周剖杀小鼠

计算减蚴率;取脾并分离脾细胞

用ConA刺激培养脾细胞

流式细胞仪检测脾细胞的凋亡发生率

同时设有BCG和PBS对照。结果:pIL-12质粒DNA免疫组的减蚴率为44.85%

脾细胞凋亡发生率明显低于PBS对照组。结论:pIL-12质粒DNA可抑制细粒棘球蚴感染小鼠的脾细胞凋亡。

Abstract

Objective:To investigate the effects of pIL-12 plasmid DNA on apoptosis of splenocytes in mice infected with protoscoleces of hydatid cyst of E.granulosus(Eg).Methods:BALB/c mice were intramuscularly vaccinated by pIL-12 plasmid DNA

then challenged with Eg protoscoleces on 8 w of vaccination and killed on 18W of infection to count rate of reduced hydatid cyst weight.The spleen was gathered to separate splenocytes

splenocytes then were cultured by stimulation with ConA

and apoptotic rate of splenocytes were measured by FACsort.BCG and PBS were served as control.Results:In the group of pIL-12 plasmid DNA immunization

the rate of reduced hydatid cyst weight was 44.85%;apoptotic rate of splenocytes in the pIL-12 plasmid DNA group was lower than that in the PBS control.Conclu-sion:pIL-12 plasmid DNA may inhibit apoptosis of splenocytes in mice infected with hydatid cyst of Echinococcus granulosus.

关键词

细粒棘球绦虫pIL-12质粒DNA凋亡

Keywords

Echinococcus granulosuspIL-12 plasmid DNAapoptosis

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