Objective:To evaluate the expression and significance of Bcl-2

Bad gene and its relationship with prognosis of breast cancer.Methods:Immunohistochemical technique was used to detect the expression of Bcl-2

Bad gene in 10 cases of normal breast tissue

10 breast fibroadenoma tissue

40 young human breast carcinoma tissue and 40 old human breast carcinoma tissue.The expression of ER and PR in 80 cases of human breast carcinoma tissue were also detected.Results:The expression of bcl-2

bad gene decreased in the development of breast cancer(P<0.01).The expressions of bcl-2

bad gene in young human breast carcinoma tissue were lower than that in old human breast carcinoma tissue(P<0.05).There was a positive correlation between the positive rate of bcl-2 and the positive rate of ER、PR(P<0.05).There was a negative correlation between the positive rate bcl-2 and histologic grade or the node metastasis(P<0.05).The expressions of bcl-2、bad gene in young human breast carcinoma tissue were lower than in old human breast carcinoma tissue(P<0.05).The expression of bad gene was not related to the expression of ER

PR

histologic grade and the node metastasis(P>0.05).Conclusion:The expression of bcl-2 and bad can be used as predicted factors of breast cancer.Detection of the expression level of the bcl-2 protein

in particular the combined detection of the expressions of bcl-2 and bad as well as ER and PR is helpful in forecasting the prognosis of breast carcinoma and instructing the treatment.