Objective:To investigate the relationship of CXCR4

VEGF-C and SDF-1 in human laryngeal carcinoma and discuss the significance of the three indexs in lymph node metastasis.Methods:The expression of CXCR4

VEGF-C and SDF-1 was detected by reverse transcription polymerase chain reaction(RT-PCR)and immuno-histochemical SP in 10 cases of adjacent normal tissue

45 cases of laryngeal carcinoma tissue and cervical lymph node tissue.Results:Not in the mRNA but the level of proteins

The positive rate of the expression of CXCR4 and VEGF-C were 78%

67%and 78%

71%in primary tumor cells

were altogether higher than that in adjacent normal tissue(P<0.05);The expreession of SDF-1 in metastasis lymph node tissue(100%)was higher than that in nonmetastasis lymph node tissue(30%).There was significant correlation between CXCR4 and VEGF-C expression in the laryngeal carcinoma(P<0.05);The expression of CXCR4 and VEGF-C in laryngeal carcinoma was significantly related to clinical TNM stage、tumor cell differentiation and lymph nodes metastasis;The expression of SDF-1 in lymph node showed significant relationship only with the state of lymph nodes metastasis(P<0.05);The correlation between the expression of CXCR4 in laryngeal carcinoma and SDF-1 in lymph node had significantly negtive correlation(r=-0.330 9

P=0.016 7).Conclusion:The overexpression of SDF-1-CXCR4-VEGF-C in laryngeal carcinoma and metastasis lymph nodes had significantly related to lymph nodes metastasis.The co-expression of CXCR4

VEGF-C

SDF-1 may play cooperation role in lymphatic metastasis of laryngeal carcinoma.