结直肠癌细胞系肿瘤干细胞相关亚群初步研究

胡祥; 程勇; 王吉明; 刘彬

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结直肠癌细胞系肿瘤干细胞相关亚群初步研究

论著 | 更新时间：2024-01-17

- 结直肠癌细胞系肿瘤干细胞相关亚群初步研究
- Preliminary study on cancer stem-like cells(side population)in human colorectal cancer cell lines
- 重庆医科大学学报 2008年33卷第12期页码：1440-1444
- 作者机构：
  
  重庆医科大学附属第一医院胃肠外科
- 作者简介：
- 基金信息：
  
  重庆市自然科学基金资助项目（CSTC,2008BB5230）
- DOI：
  中图分类号： R735.3
- 纸质出版日期：2008，
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[1]胡祥,程勇,王吉明,刘彬.结直肠癌细胞系肿瘤干细胞相关亚群初步研究[J].重庆医科大学学报,2008,33(12):1440-1444.

HU Xiang, et al. Preliminary study on cancer stem-like cells(side population)in human colorectal cancer cell lines[J]. Journal of Chongqing Medical University, 2008,33(12):1440-1444.
[1]胡祥,程勇,王吉明,刘彬.结直肠癌细胞系肿瘤干细胞相关亚群初步研究[J].重庆医科大学学报,2008,33(12):1440-1444. DOI：

HU Xiang, et al. Preliminary study on cancer stem-like cells(side population)in human colorectal cancer cell lines[J]. Journal of Chongqing Medical University, 2008,33(12):1440-1444. DOI：

摘要

目的:检测不同结直肠癌细胞系中存在肿瘤干细胞样亚群

探讨初步富集肿瘤干细胞相关亚群的方法。方法:采用流式细胞术检测结直肠癌细胞系LOVO、SW480、HCT116中的侧群细胞（Side population

SP）细胞

添加生长因子的无血清培养基（Serum-free medium

SFM）培养SW480初步分离

富集肿瘤干细胞样亚群

有限稀释实验

诱导分化

传代更新

含血清/无血清培养基交替培养证实肿瘤干细胞相关亚群的存在。结果:LOVO、SW480、HCT116细胞系中含SP细胞分别为1.0%

1.2%和1.3%;SW480细胞中约含有0.54%￣0.62%的细胞能够在无血清培养基中能够存活、增殖

形成悬浮的肿瘤细胞球;在SFM中加入血清可促使肿瘤干细胞相关细胞分化;肿瘤干细胞相关细胞可以连续传代

并可交替培养于含10%小牛血清培养基（Serum-supplied medium

SSM）和SFM中。结论:结直肠癌细胞系LOVO、SW480、HCT116中含SP细胞

SW480中的肿瘤干细胞相关亚群可以无血清培养基富集。

Abstract

Objective:To investigate the content of cancer stem-like cells in different human colorectal cancer ce11 lines and explore the methods for enriching cancer stem-like cells in SW480.Methods:The percentage of side population cells(SP)in human colorectal cancer cell lines LOVO

SW480

HCT116 were detected by flow cytometry.SW480 cell line was cultivated in serum-free medium(SFM)supplemented with growth factors and cancer stem-like cells reforming into floatingspheres were isolated.The isolated cancer stem-like cells were identified by limiting-dilution assay

differentiation assay

self-renewal assay

alternative cultivation assay.Results:The percentage of SP cells was 1.0% in LOVO

1.2% in SW480 and 1.3% in HCT116.In the absence of serum

a minority(0.54%￣0.62%)of cancer stem-like cells in SW480 cells survived

proliferated and assembled into the suspended tumor cell spheres.SW480 cancer stem-like cells possessed proliferative

self-renewal and differentiation potential

which were responsible for the floating tumor clone.Serum addition into SFM resulted in the proliferation of cancer stem-like cells;after several generations and alternated cultivation in SSM and SFM

cancer stem-like cells maintained their characters.Conclusion:LOVO

SW480 and HCT116 cell lines contain a tiny minority of SP cells with stem cell properties.The cancer stem-like cells in SW480 line can be maintained in SFM using a floating culture method.

关键词

结直肠癌细胞系肿瘤干细胞

Keywords

colorectal cancerCell linecancer stem cell

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