Objective:To investigate the proliferation inhibitory effect of crocin on transitional cell carcinoma of urinary bladde(rTCCB). Methods:MTT assay was used to evaluate the inhibitory effect of Crocin on the proliferation of T24 cells. Microscope was used to observe the morphological changes of the T24 cells. Flow cytometry was used to measure the cell cycle. T24 cells were inoculated in to BALB/c nude mice to establish bladder cancer model. After treatment with Crocin

the inhibitory effects were observed on the growth of tumors. Results:The proliferation of T24 cells were inhibited remarkably. Morphological changes of cells were observed. Flow cytometric profiles revealed that crocin led to the increase of the cells in G0/G1 phase and the decrease of cells in S phase and G2/M(P<0.05)

Crocin could inhibit the growth of BALB/c xenograft tumor significant r. The inhibitory rate of tumor was 28.7%. Conclusion:Crocin exerts an inhibitory effect on the growth of transitional cell carcinoma of urinary bladder in vitro and vivo.