Objective:Toinvestigate the effects ofantisense oligonucleotide(ASODN)targetinghsa-miR-518b on the growth and cycle of gastric cancer cell

and the mechanism in gastric cancer.Methods:Specific phosphorothioate antisense oligodeoxynucleotides targeting hsa-miR-518b were synthesized and then transfected into the gastric cancer cel(lSGC7901).We investigated the change of the hsa-miR-518b expression in SGC7901 after the transfection by using real time RT-PCR.Methyl thiazolyl tetrazolium(MTT)assay was used to detect the cell growth of SGC 7901 and fluorescence activated cell sorting(FACS)was used to analyze the cell cycle of SGC7901.Results:After transfection

the expression of hsa-miR-518b was decrased(P=0.000)

the growth of SGC7901 was suppressed(P=0.000) and the cell cycle arrested in G(1P=0.000).Conclusion:hsa-miR-518b(ASODN)could suppress the growth of SGC 7901 by arresting the cell cycle in G1 phase.Hsa-miR-518b could promote the growth of gastric cancer cell by regulating Gl/S transition.