Objective:To investigate the role of Danshensu on TGFβ1 stimulated signal transduction in rat hepatic stellate cells(HSCs).Methods:The mice HSCs were isolated and cultured for the following study.HSC were treated with different concentration Danshensu(0.062 5～0.250 0 mmol/L)and TGFβ1 to detect the proliferation、α-SMA protein expression and the chang of Smad2

Smad3

Smad7 mRNA.Results(:1)Danshensu in the concentration of 0.062 5～0.250 0 mmol/L could prevent the proliferation of HSC induced by TGFβ1 in a dose-dependent manner(P<0.05)(.2)The concentration of 0.250 0 mmol/L Danshensu could downregulateα-SMA protein expression at 48th hour in HSC

with or without stimulation of TGFβ1(P<0.01)

and the activation of HSC was inhibited also.(3)TGFβ1 increased the mRNA level of Smad2

3

7 in HSC;Danshensu could downregulate the mRNA level of Smad2

3 and upregulate the mRNA levelofSmad7 in HSC induced by TGFβ1(P<0.01).Conclusion:Danshensu could restore the normal transmembrane signal of TGFβ1 through upregulation of Smad7 mRNA expression and downregulation of Smad2

Smad3 mRNA expression in HSC.