咪喹莫特诱导多品系小鼠脱毛动物模型的建立

杜佳; 赵恒光; 李惠

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咪喹莫特诱导多品系小鼠脱毛动物模型的建立

技术方法 | 更新时间：2024-01-17

- 咪喹莫特诱导多品系小鼠脱毛动物模型的建立
- Establishment of alopecia animal model induced by imiquimod in multi-strain mice
- 重庆医科大学学报 2009年34卷第3期页码：307-309
- 作者机构：
  
  重庆医科大学附属第一医院皮肤科
- 作者简介：
- 基金信息：
  
  重庆市科委基金资助项目（2007BB5296）
- DOI：
  中图分类号： R-332
- 纸质出版日期：2009，
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[1]杜佳,赵恒光,李惠.咪喹莫特诱导多品系小鼠脱毛动物模型的建立[J].重庆医科大学学报,2009,34(03):307-309.

DU Jia, et al. Establishment of alopecia animal model induced by imiquimod in multi-strain mice[J]. Journal of Chongqing Medical University, 2009,34(3):307-309.
[1]杜佳,赵恒光,李惠.咪喹莫特诱导多品系小鼠脱毛动物模型的建立[J].重庆医科大学学报,2009,34(03):307-309. DOI：

DU Jia, et al. Establishment of alopecia animal model induced by imiquimod in multi-strain mice[J]. Journal of Chongqing Medical University, 2009,34(3):307-309. DOI：

摘要

目的:研究咪喹莫特外用建立多品系小鼠脱毛动物模型的可行性及其初步机理。方法:BARB/c、129、C3H/HeJ、C574种品系小鼠各30只

各品系鼠均随机分成两组

实验组4组*15只

背部外用咪喹莫特每周3次;对照组4组*15只

外用不含药物的空白基质

随访一定时间后观察脱毛结果

并用免疫组化法检测毛囊周围TLR7受体的分布。结果:四种品系小鼠在给药7～10d后均出现脱毛现象

形成斑片状脱毛区

成功率100%。TLR7受体在各品系小鼠的毛囊及其周围均有强阳性表达。结论:咪喹莫特外用能稳定地诱导BARB/c、129、C3H/HeJ、C57品系小鼠建立脱毛动物模型

是一种非疤痕性、非炎症性脱发

其机理可能与咪喹莫特诱导激活毛囊周围的TLR7受体有关。

Abstract

Objectives:To establish a novel alopecia animal model in multi-strain mice.Methods:BARB/c mice

129 mice

C57mice and C3H/HeJ mice were purchased at the age of 6 to 8 weeks. 30 mice of each kind were divided into control and experimental groups randomly. The control group were treated with vehicle alone. The experimental groups were treated with imiquimod cream with cotton swaps for back skin 3 times a week. The existence of TLR7 on skin was examined by immunohistochemistry(IHC). Results:Alopecia lesions were observed in all the mice in experimental group

but mice in control group could not induce by imiquimod. The aggregation of TLR7were observed in hair follicle. Conclusions: Imiquimod can stably induce non-inflammatory alopecia in BARB/c mice

129 mice

C57mice and C3H/HeJ mice by activating TLR7.

关键词

咪喹莫特Toll样受体7脱毛

Keywords

imiquimodToll-likereceptor7Alopecia

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