Objectives:To compare the transcriptional activities of tumor-specific hTERT promoters and survivin promoter in human lung cancer A549 cells

in order to lay some groundwork for targeting gene therapy in human lung cancer.Methods:The 1 084 bp fragment of hTERT promoter and 980bp survivin promoter from A549 cells were acquired by PCR amplification.The eukaryotic expression plasmids(pGL-H-RED

pGL-LH-RED and pGL-S-RED)which have Red Fluorescin reporter gen(eRed)and are modulated by 263 bp hTERT promoter

1084bp hTERT promoter and 980bp survivin promoter respectively were constructed based on the pGL3-hTERT which has 263bp hTERT promoter from HepG2 cells.These recombinant plsmids were transfected into A549 and MRC-5 cells with liposome.After 72 hours

the expression level of red fluorescin target protein modulated by 3 promoters at the same time was analyzed by Imagepro-Plus6.0 software.Results:The red fluorescin target protein was observed in transfected A549 cells

but there was no red fluorescence in transfected MRC-5 control cells.The pGL-S-RED had the highest expression activity in A549 cells and the strength rate(IOD)of red fluorescence of A549 cells transfected by pGL-S-RED

pGL-LH-RED and pGL-H-RED were 201.17

171.70

and 136.34 respectively.Conclusion:Our data reveals that the cloned hTERT and survivin promoter are tumor-specific

and the survivin promoter has the highest transcriptional activities in lung cancer A549 cells

and may serve as a useful tool for transcriptional targeting gene therapy of human lung cancer.