Objective:To evaluate the effect and mechanism ofintraoperative regional arterial infusion chemotherapy(RAIC)on gastric cancer. Methods: The expressions of Ki-67 and VEGF

in 30 cases of gastric cancer

before and after the treatment of intraoperative RAIC

were shown by SP immunohistochemical staining. And pathological changes of tumor were detected. Results:After the intraoperative RAIC

the expressions of Ki-67 and VEGF were significantly lower than those before treatmen(tP<0.05).Light microscope examination revealed a mild change of karyopyknosis

nuclear swelling

coagulation of cytoplasm in cancer cells

mild hydropsia of intercellular substance

invasion of inflammatory cells and mild vasculitis in some cases. Transmission electron microscope found that nuclear swelling

nuclear heterochromatin agglutination

nuclear-week gap expansion

mitochondrial swelling

endoplasmic reticulum expansion

and Golgi complex expansion.Conclusion:The intraoperative RAIC for gastric cancer could obviously depress the expressions of Ki-67 and VEGF and cause the histo-pathological changes of cancer cells. Intra operative RAIC could significantly inhibit invasion activity of cancer cells and reduce iatrogenic metastasis and post operative recurrence rate.