Objective:To evaluate the synergism ofrecombinant M.smegmatis(MS)encoding human granulysin and murine IL-12 to anti-tuberculosis chemotherapy in murine M.tuberculosis M.tuberculosis infection.Methods:Balb/c mice were infected with normal saline

recombinant MS

INH(Isoniazid)+PZA(pyrazinamide)

recombinant MS+INH+PZA respectively.The numbers of viable bacteria in the lung and the spleen were counted.The levels of IL12 and IFN-γin serum and IFN-γand TNF-αreleased from spleen lymphocytes stimulated with PPD were detected with ELISA.Lungs and spleens were prepared for pathological analysis.Results:The recombinant MS group

INH+PZA group

and recombinant MS+INH+PZA group showed significantly reduced number of colony forming units(log CFU/g) compared with saline group.The increase of IL-12 and IFN-γin serum were found in recombinant MS group.IFN-γand TNF-αreleased from spleen lymphocytes stimulated with PPD in recombinant MS group was higher than that in other groups(P<0.01).The pathological changes in lungs of the recombinant MS group

INH(Isoniazid)+PZA(pyrazinamide)group

and recombinant MS+INH+PZA group were localized

while those in the control group were extensive.The significant pathological changes were not found in the spleens of all groups.Conclusion:The recombinant MS had immunotherapeutic effects

the recombinant MS had a synergism to anti-tuberculosis chemotherapy.