Objective:To investigate the expression of PLCζin INS-1

and the role of PLCζin glucose-stimulated insulin secretion(GSIS).Methods:(1)The six primers of PLC isoforms were designed.The template of INS-1’s RNA was collected

RT-PCR was used to analyse the expression of PLC isoforms.(2)The glucose concentration were set at: 10

20

40

80

100 mmol/L

and stimulated INS-1 respectively.The content of insulin in INS-1’s supernatant liquid was detected.The time groups were set as: 20

40

60

80

120 min.The content of insulin in INS-1’s supernatant liquid was detected.(3)After the stimulus of glucose

RT-PCR and Western Blot were used to detect the expression of PLCζ.Results:(1) PLCβ

PLCγ

PLCδ

PLCζ

PLCηwere expressed in INS-1.(2) Exposure of INS-1 to 40mM glucose for 60 min

the content of insulin secretion reached the maximum and RT-PCR and Western Blot detected that the expression of PLCζwas raised.Conclusion:(1)PLCζwas expressed in INS-1.(2)Under the stimulation of glucose

the PLCζ’s expression was augmented

so PLCζ was probably participated in the signal transduction pathway of GSIS in INS-1.