Objective:To study the effect of a new medical biomaterial—bfgf-fibrin glue-amniotic membrane(bFAM) for corneal alkali burn and provide experimental foundation for the clinical application in the future. Methods: We use fibrin glue compounded with bFGF and amniotic membrane to prepare the bFAM. 45 New Zealand rabbits were used to make corneal alkali burn models and divided into 3 groups:bFAM group、amniotic membrane group、comparison group

each group have 15 rabbits. After operation

we observe the linical curative effect. 7d、14d and 28d after operation

5 corneas were extracted for histopathological analysis. The expression of bFGF in corneas was measured by immunohistochemistry analysis. Results: After operation

bFAM group have better curative effect than amniotic membrane group in Inflammatory reaction、tissue repair and anti-CNV. The expression of bFGF in bFAM group is much higher than amniotic membrane group and comparison group in the day 7 afer operation

and in other time points the expression have not distinct difference in all groups. Conclusion: Compared with single layer amniotic membrane

bFAM shows better curative effect for corneal alkali burn.