Objective:We established nude mouse model of laryngocarcinoma and detect the disseminated tumor cell of peripheral blood and mutant p53 in tumour tissue

to research the correlation of DTC with variant p53 gene and their value of predicting cancer metastasis and prognosis in laryngocarcinoma.Methods:There are 40 nude mice in the experimental group which accepted hypodermic injection of laryngocarcinoma cell

10 in control.By the reverse transcription polymerase chain reaction and nested polymerase chain reaction(Nest-PCR)

we tested CK19mRNA in nude mouse’s peripheral blood

as a marker of disseminated cancer cell at the 10th weeks after tumor-planting.We also tested the expression of p53 gene in tumour tissue by RT-PCR and immunohistology.After the nude dead

we dissect lung

liver and lymph node and use continuous pathological dyeing confirmed whether the transfer.Results:The positive rate of DTC was 50.0%(20/40)

but negative in the control group

the difference was significant(P<0.05).In tumor tissue

the positive rate of mutant p53 gene was 55.0%(22/40);The expression of DTC was positive correlation with p53.The mean one-year the mean survival time of DTC and p53 positive group was 34 weeks vs 43 weeks in negative group

the difference was significant(P<0.05).The positive of group have distant metastasis(38.9%)

but in negative group only(12.5%)

the difference was significant(P<0.05).Conclusion:The expression of DTC associated with variant p53 protein could be used as an indicator of tumor metastasis

and it might be an independent risk factor for an unfavourable prognosis;DTC was positive correlation with variant p53 protein.