Objective:To investigate the effect of inhibition of vascular endothelial growth factor(VEGF) antisense oligodeoxynucleotides(ASODN) on human bladder carcinoma in nude mice mediated by ultrasound microbubble. Methods:The mice were randomly divided into 3 groups when transplantation tumor was established: For UM+ASODN group

mixture of microbubble and VEGF-ASODN was inject into every mice from caudal vein plus ultrasound tumor local irradiation. For UM group

microbubble was inject into very mice from caudal vein plus local irradiation with the same condition. For control group

physiological saline was inject into every mice from caudal vein.The treatment was performed every 3 days with total 5 times.The volume change of mouse subcutane tumors was observed.Evaluate the expression of VEGF and CD34 and Ki67 with immunohistochemistry dyeing and calculate the microvessel density (MVD) and cell proliferation index;TUNEL assay was used to analyse the apoptosis of tumor cells. Results:The growth speed of nude mice tumor in UM+ASODN group was obviously slower than control group; The expression of VEGF and The MVD in UM+ ASODN group was lower than control group(P<0.05); To contrast with control group

the apoptosis of tumor in UM+ ASODN group was increased(P<0.01)

but the proliferate of tumor in UM+ ASODN group was decreased(P<0.05). Conclusion:VEGF antisense oligonucleotides mediated by ultrasound microbubbe can efficiently inhibit the growth of human bladder carcinoma in nude mice.